primary antibodies against ccl2  (Novus Biologicals)

Journal: Cancer Research Communications

Article Title: Defining the Ovarian Cancer Precancerous Landscape through Modeling Fallopian Tube Epithelium Reprogramming Driven by Extracellular Vesicles

doi: 10.1158/2767-9764.CRC-25-0064

Figure Lengend Snippet: Validation of 1-day and 14-day treatment transcriptomics data using IHC. A, Left, CCL2 transcript expression in OVCAR3-, FT240-, and PBS-treated secretory cells after 1 day. Right, IHC staining for upregulated CCL2 in FTE from three individual patients. B, Left, FLNA transcript expression in OVCAR3-, FT240-, and PBS-treated secretory cells after 1 day. Right, IHC staining for downregulated FLNA in FTE from three individual patients. C, Left, VCAM1 transcript expression in OVCAR3-, FT240-, and PBS-treated secretory cells after 1 day. Right, IHC staining for upregulated VCAM1 in FTE from three individual patients. D, Left, TPI1 transcript expression in OVCAR3-, FT240-, PBS-treated secretory cells after 1 day. Right, IHC staining for downregulated protein TPI1 in FTE from three individual patients. E, Left, TXNIP transcript expression in OVCAR3-, FT240-, and PBS-treated secretory cells after 14 days. Right, IHC staining for downregulated TXNIP in FTE from three individual patients. F, Left, VCAM1 transcript expression in OVCAR3-, FT240-, and PBS-treated ciliated cells after 14 days. Right, IHC staining for upregulated VCAM1 in FTE from three individual patients. In all boxplots, each point represents normalized transcript abundance measured in a single segment. Scale bar, 20 μm; arrows indicate epithelium.

Article Snippet: Primary antibodies against CCL2 (Novus, cat. #NBP1-07035SS, RRID: AB_1625611), VCAM1 (Thermo Fisher Scientific, cat. #MA5-31965, RRID: AB_2809259), FLNA (Proteintech, cat. #67133-1-Ig, RRID: AB_2882432), TPI1 (Proteintech, cat. #10713-1-AP, RRID: AB_2207716), and TXNIP (Thermo Fisher Scientific, cat. #40-3700, RRID: AB_2533462) were incubated overnight at 4°C, followed by detection using a horseradish peroxidase–linked secondary antibody and DAB substrate (the full procedure is provided in the Supplementary Methods).

Techniques: Biomarker Discovery, Expressing, Immunohistochemistry

Journal: PeerJ

Article Title: Identification and validation of three diagnostic autophagy-related genes associated with advanced plaques and immune cell infiltration in carotid atherosclerosis based on integrated bioinformatics analyses

doi: 10.7717/peerj.18543

Figure Lengend Snippet: ROC curves of (A) CCL2, (B) LAMP2, and (C) CTSB. (D) The GSE28829 dataset was employed as an external validation sample. (E) Logistic regression model combining three key CAS-related DE-ATGs in GSE100927 . (F) Logistic regression model combining three key CAS-related DE-ATGs in GSE28829 .

Article Snippet: Subsequently, the membranes were incubated overnight at 4 °C with the following rabbit polyclonal primary antibodies against CCL2 (A7277; Abclonal, Woburn, MA, USA), LAMP2 (ab199947; Abcam, Cambridge, UK), CTSB (12216-1-AP; Proteintech, Rosemont, IL, USA), and β-actin (20536-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Biomarker Discovery

Journal: PeerJ

Article Title: Identification and validation of three diagnostic autophagy-related genes associated with advanced plaques and immune cell infiltration in carotid atherosclerosis based on integrated bioinformatics analyses

doi: 10.7717/peerj.18543

Figure Lengend Snippet: (A) HUVECs and HAoSMCs were incubated with ox-LDL (50 μg/mL) for 24 h or with normal medium for 24 h, and the expression of CCL2, LAMP2, and CTSB was measured by western blot (original blots are presented in and ). (B) Quantitative result of western blot. (C) QRT-PCR measured the expression of CCL2, LAMP2, and CTSB. (D) ORO staining of the entire aortas. Scale bars, 2 mm. (E) HE staining of the aortic root. Scale bars, 200 µm. (F) The aortic root was stained with antibodies against CCL2, LAMP2, and CSTB. Scale bars, 100 µm. (G) Quantification of the proportion of ORO positive area/total aortic area (%). (H) Quantitative data of the aortic root plaque area. (I–K) The percentages of CCL2, LAMP2, and CTSB positive area in the aortic root lesion. Data are presented as mean ± SD ( n = 6). * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: Subsequently, the membranes were incubated overnight at 4 °C with the following rabbit polyclonal primary antibodies against CCL2 (A7277; Abclonal, Woburn, MA, USA), LAMP2 (ab199947; Abcam, Cambridge, UK), CTSB (12216-1-AP; Proteintech, Rosemont, IL, USA), and β-actin (20536-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Incubation, Expressing, Western Blot, Quantitative RT-PCR, Staining

Journal: PeerJ

Article Title: Identification and validation of three diagnostic autophagy-related genes associated with advanced plaques and immune cell infiltration in carotid atherosclerosis based on integrated bioinformatics analyses

doi: 10.7717/peerj.18543

Figure Lengend Snippet: ROC curves of (A) CCL2, (B) LAMP2, and (C) CTSB. (D) The GSE28829 dataset was employed as an external validation sample. (E) Logistic regression model combining three key CAS-related DE-ATGs in GSE100927 . (F) Logistic regression model combining three key CAS-related DE-ATGs in GSE28829 .

Article Snippet: The sections were incubated with rabbit polyclonal primary antibodies against CCL2 (A7277; Abclonal, Woburn, MA, USA), LAMP2 (ab199947; Abcam, Cambridge, UK), and CTSB (12216-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Biomarker Discovery

Journal: PeerJ

Article Title: Identification and validation of three diagnostic autophagy-related genes associated with advanced plaques and immune cell infiltration in carotid atherosclerosis based on integrated bioinformatics analyses

doi: 10.7717/peerj.18543

Figure Lengend Snippet: (A) HUVECs and HAoSMCs were incubated with ox-LDL (50 μg/mL) for 24 h or with normal medium for 24 h, and the expression of CCL2, LAMP2, and CTSB was measured by western blot (original blots are presented in and ). (B) Quantitative result of western blot. (C) QRT-PCR measured the expression of CCL2, LAMP2, and CTSB. (D) ORO staining of the entire aortas. Scale bars, 2 mm. (E) HE staining of the aortic root. Scale bars, 200 µm. (F) The aortic root was stained with antibodies against CCL2, LAMP2, and CSTB. Scale bars, 100 µm. (G) Quantification of the proportion of ORO positive area/total aortic area (%). (H) Quantitative data of the aortic root plaque area. (I–K) The percentages of CCL2, LAMP2, and CTSB positive area in the aortic root lesion. Data are presented as mean ± SD ( n = 6). * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: The sections were incubated with rabbit polyclonal primary antibodies against CCL2 (A7277; Abclonal, Woburn, MA, USA), LAMP2 (ab199947; Abcam, Cambridge, UK), and CTSB (12216-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Incubation, Expressing, Western Blot, Quantitative RT-PCR, Staining